Try applying the animation to both an R6 and R15 rig by making both rigs and going to ••• —> Import —> From Roblox and checking which rig actually makes the animation play
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If you play the animation multiple times, does it play? I’m thinking that maybe if the animation is short that the animation will “play” before loading fully
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It is a r15 animation. The r6 does not work.
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and is your game r15 as well? (ignore)
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It’s supposed to only plays when I use a tool. It does not work at all right now.
No but my character is r15. (Ignore)
I think this has something to do with roblox studio and not your game. you could maybe test that out, can you insert a script into a dummy which plays that animation in a loop, and then run (Not play) the game and check if the animation is working on that dummy. My animation somehow only worked that way and not when I played it.
Does not play on dummy either. Im so confused 
Did you set the animation priority? If not, try using this line of code to define it: AnimationTrack.Priority = Enum.AnimationPriority.Action -- change this to whatever
Oh yeah this is definitely a good thing to check; if this hasn’t been checked yet then I’m not sure how it missed so many commenters. Make sure you actually set the priority in the animation itself using the animation priority menu. Setting animation properties during runtime is unstable.