If you want to write more lines, move the disconnect function to the end of the code:
local TS = game:GetService("Tweenservice")
local TI = TweenInfo.new(1)
local Event
Event = game:GetService("UserInputService").InputBegan:Connect(function(inputObject, gameProcessedEvent)
local Animation = TS:Create(script.Parent.Title, TI {Position = where you want the frame to be})
Animation:Play()
Animation.Completed:Wait()
--More code here
Enent:Disconnect() -- Disconnect at the end of code
end)