Is the datastore In studio different than the one in Player?

When I am testing in studio and I change the datastore, does it apply for Roblox Player after I publish the game?

Yes, it should transition from studio to the game

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Writing to the Datastores in studio will only update the live game if you have the API enabled:

https://developer.roblox.com/en-us/articles/Data-store

Otherwise it will not change the data in the Live game.